PT  - JOURNAL ARTICLE
AU  - Makoto Akashi
AU  - Naoto Hayasaka
AU  - Shin Yamazaki
AU  - Koichi Node
TI  - Mitogen-Activated Protein Kinase Is a Functional Component of the Autonomous Circadian System in the Suprachiasmatic Nucleus
AID  - 10.1523/JNEUROSCI.3410-07.2008
DP  - 2008 Apr 30
TA  - The Journal of Neuroscience
PG  - 4619--4623
VI  - 28
IP  - 18
4099  - http://www.jneurosci.org/content/28/18/4619.short
4100  - http://www.jneurosci.org/content/28/18/4619.full
SO  - J. Neurosci.2008 Apr 30; 28
AB  - The suprachiasmatic nucleus (SCN) is the master circadian pacemaker driving behavioral and physiological rhythms in mammals. Circadian activation of mitogen-activated protein kinase [MAPK; also known as ERK (extracellular signal-regulated kinase)] is observed in vivo in the SCN under constant darkness, although the biological significance of this remains unclear. To elucidate this question, we first examined whether MAPK was autonomously activated in ex vivo SCN slices. Moreover, we investigated the effect of MAPK inhibition on circadian clock gene expression and neuronal firing rhythms using SCN-slice culture systems. We show herein that MAPK is autonomously activated in the SCN, and our data demonstrate that inhibition of the MAPK activity results in dampened rhythms and reduced basal levels in circadian clock gene expression at the SCN single-neuron level. Furthermore, MAPK inhibition attenuates autonomous circadian neuronal firing rhythms in the SCN. Thus, our data suggest that light-independent MAPK activity contributes to the robustness of the SCN autonomous circadian system.