PT  - JOURNAL ARTICLE
AU  - Fu-Wen Zhou
AU  - Shannon G. Matta
AU  - Fu-Ming Zhou
TI  - Constitutively Active TRPC3 Channels Regulate Basal Ganglia Output Neurons
AID  - 10.1523/JNEUROSCI.3978-07.2008
DP  - 2008 Jan 09
TA  - The Journal of Neuroscience
PG  - 473--482
VI  - 28
IP  - 2
4099  - http://www.jneurosci.org/content/28/2/473.short
4100  - http://www.jneurosci.org/content/28/2/473.full
SO  - J. Neurosci.2008 Jan 09; 28
AB  - A hallmark of the GABA projection neurons of the substantia nigra pars reticulata (SNr), a key basal ganglia output nucleus, is its depolarized membrane potential and rapid spontaneous spikes that encode the basal ganglia output. Parkinsonian movement disorders are often associated with abnormalities in SNr GABA neuron firing intensity and/or pattern. A fundamental question remains regarding the molecular identity of the ion channels that drive these neurons to a depolarized membrane potential. We show here that SNr GABA projection neurons selectively express type 3 canonical transient receptor potential (TRPC3) channels. These channels are tonically active and mediate an inward, Na+-dependent current, leading to a substantial depolarization in these neurons. Inhibition of TRPC3 channels induces hyperpolarization, decreases firing frequency, and increases firing irregularity. These data demonstrate that TRPC3 channels play important roles in ensuring the appropriate firing intensity and pattern in SNr GABA projection neurons that are crucial to movement control.