PT  - JOURNAL ARTICLE
AU  - Cecilia Bouzat
AU  - Mariana Bartos
AU  - Jeremías Corradi
AU  - Steven M. Sine
TI  - The Interface between Extracellular and Transmembrane Domains of Homomeric Cys-Loop Receptors Governs Open-Channel Lifetime and Rate of Desensitization
AID  - 10.1523/JNEUROSCI.0448-08.2008
DP  - 2008 Jul 30
TA  - The Journal of Neuroscience
PG  - 7808--7819
VI  - 28
IP  - 31
4099  - http://www.jneurosci.org/content/28/31/7808.short
4100  - http://www.jneurosci.org/content/28/31/7808.full
SO  - J. Neurosci.2008 Jul 30; 28
AB  - The lifetimes of activated postsynaptic receptor channels contribute to the efficiency of synaptic transmission. Here we show that structural differences within the interface dividing extracellular and transmembrane domains of homomeric α7 and 5-HT3A receptors account for the large differences in open-channel lifetime and time of desensitization onset between these contrasting members of the Cys-loop receptor superfamily. For α7 receptors, agonist-evoked single-channel currents appear mainly as isolated brief openings (τo = 0.35 ms), whereas macroscopic currents after a step pulse of agonist desensitize rapidly (τd = 0.4 ms). In contrast for 5-HT3A receptors, agonist-evoked single-channel currents appear as clusters of many long openings in quick succession (τcluster = 1.2 s), whereas macroscopic currents desensitize slowly (τd = 1.1 s). A chimeric α7-5HT3A receptor exhibits functional properties intermediate between those of the parent receptors, but the functional signatures of each parent are reconstituted after substituting the major loops within the interface of the extracellular and transmembrane domains from the corresponding parent receptor. Furthermore, these structural loops contribute to open-channel lifetime and time of desensitization onset in a nonadditive manner. The results suggest that desensitization is the major determinant of the lifetimes of activated α7 and 5-HT3A receptors and that functional differences between the two receptors arise primarily through structural differences at the interface between extracellular and transmembrane domains.