PT  - JOURNAL ARTICLE
AU  - Genevíève Gowing
AU  - Thomas Philips
AU  - Bart Van Wijmeersch
AU  - Jean-Nicolas Audet
AU  - Maarten Dewil
AU  - Ludo Van Den Bosch
AU  - An D. Billiau
AU  - Wim Robberecht
AU  - Jean-Pierre Julien
TI  - Ablation of Proliferating Microglia Does Not Affect Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Caused by Mutant Superoxide Dismutase
AID  - 10.1523/JNEUROSCI.3494-08.2008
DP  - 2008 Oct 08
TA  - The Journal of Neuroscience
PG  - 10234--10244
VI  - 28
IP  - 41
4099  - http://www.jneurosci.org/content/28/41/10234.short
4100  - http://www.jneurosci.org/content/28/41/10234.full
SO  - J. Neurosci.2008 Oct 08; 28
AB  - Microglial activation is a hallmark of all neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). Here, a detailed characterization of the microglial cell population within the spinal cord of a mouse model of familial ALS was performed. Using flow cytometry, we detected three distinct microglial populations within the spinal cord of mice overexpressing mutant superoxide dismutase (SOD1): mature microglial cells (CD11b+, CD45low), myeloid precursor cells (CD11b+, CD45int), and macrophages (CD11b+, CD45high). Characterization of cell proliferation within the CNS of SOD1G93A mice revealed that the expansion in microglial cell population is mainly attributable to the proliferation of myeloid precursor cells. To assess the contribution of proliferating microglia in motor neuron degeneration, we generated CD11b-TKmut-30; SOD1G93A doubly transgenic mice that allow the elimination of proliferating microglia on administration of ganciclovir. Surprisingly, a 50% reduction in reactive microglia specifically in the lumbar spinal cord of CD11b-TKmut-30; SOD1G93A doubly transgenic mice had no effect on motor neuron degeneration. This suggests that proliferating microglia-expressing mutant SOD1 are not central contributors of the neurodegenerative process in ALS caused by mutant SOD1.