TY - JOUR T1 - D<sub>2</sub> Dopamine Receptor Activation Facilitates Endocannabinoid-Mediated Long-Term Synaptic Depression of GABAergic Synaptic Transmission in Midbrain Dopamine Neurons via cAMP-Protein Kinase A Signaling JF - The Journal of Neuroscience JO - J. Neurosci. SP - 14018 LP - 14030 DO - 10.1523/JNEUROSCI.4035-08.2008 VL - 28 IS - 52 AU - Bin Pan AU - Cecilia J. Hillard AU - Qing-song Liu Y1 - 2008/12/24 UR - http://www.jneurosci.org/content/28/52/14018.abstract N2 - Endocannabinoid (eCB) signaling mediates short-term and long-term synaptic depression (LTD) in many brain areas. In the ventral tegmental area (VTA) and striatum, D2 dopamine receptors cooperate with group I metabotropic glutamate receptors (mGluRs) to induce eCB-mediated LTD of glutamatergic excitatory and GABAergic inhibitory (I-LTD) synaptic transmission. Because D2 receptors and group I mGluR agonists are capable of inducing the release of eCBs, the predominant hypothesis is that the cooperation between these receptors to induce eCB-mediated synaptic depression results from the combined activation of type I cannabinoid (CB1) receptors by the eCBs. By determining the downstream effectors for D2 receptor and group I mGluR activation in VTA dopamine neurons, we show that group I mGluR activation contributes to I-LTD induction by enhancing eCB release and CB1 receptor activation. However, D2 receptor activation does not enhance CB1 receptor activation, but facilitates I-LTD induction via direct inhibition of cAMP-dependent protein kinase A (PKA) signaling. We further demonstrate that cAMP/PKA signaling pathway is the downstream effector for CB1 receptors and is required for eCB-mediated I-LTD induction. Our results suggest that D2 receptors and CB1 receptors target the same downstream effector cAMP/PKA signaling pathway to induce I-LTD and D2 receptor activation facilitates eCB-mediated I-LTD in dopamine neurons not by enhancing CB1 receptor activation, but by enhancing its downstream effects. ER -