RT Journal Article
SR Electronic
T1 Long-Term Potentiation in the Rat Medial Vestibular Nuclei Depends on Locally Synthesized 17β-Estradiol
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 10779
OP 10783
DO 10.1523/JNEUROSCI.1697-09.2009
VO 29
IS 34
A1 Grassi, Silvarosa
A1 Frondaroli, Adele
A1 Dieni, Cristina
A1 Scarduzio, Mariangela
A1 Pettorossi, Vito E.
YR 2009
UL http://www.jneurosci.org/content/29/34/10779.abstract
AB In male rat brainstem slices, we investigated the involvement of locally synthesized 17β-estradiol (E2) in the induction in the medial vestibular nucleus (MVN) of long-term potentiation (LTP) by high-frequency stimulation (HFS) of the primary vestibular afferents. We demonstrated that the blockade of aromatase by letrozole or of E2 receptors (ERα and ERβ) by ICI 182,780 prevented the HFS-induced LTP of the N1 wave of the evoked field potential (FP) without affecting baseline responses. Only prolonged afferent activation could induce low LTP. In contrast, HFS applied under a combined blockade of GABAA receptors and aromatase or ERs was still able to induce LTP, but it was significantly lower and slower. These findings demonstrate that E2 does not have a tonic influence on the activity of the MVN neurons and provide the first evidence of the crucial role played by local synthesis of E2 in inducing LTP. We suggest that the synthesis of E2 occurs after aromatase activation during HFS and facilitates the development of vestibular synaptic plasticity by influencing glutamate and GABA transmission.