PT  - JOURNAL ARTICLE
AU  - Scimemi, Annalisa
AU  - Tian, Hua
AU  - Diamond, Jeffrey S.
TI  - Neuronal Transporters Regulate Glutamate Clearance, NMDA Receptor Activation, and Synaptic Plasticity in the Hippocampus
AID  - 10.1523/JNEUROSCI.4845-09.2009
DP  - 2009 Nov 18
TA  - The Journal of Neuroscience
PG  - 14581--14595
VI  - 29
IP  - 46
4099  - http://www.jneurosci.org/content/29/46/14581.short
4100  - http://www.jneurosci.org/content/29/46/14581.full
SO  - J. Neurosci.2009 Nov 18; 29
AB  - In the mammalian brain, the specificity of excitatory synaptic transmission depends on rapid diffusion of glutamate away from active synapses and the powerful uptake capacity of glutamate transporters in astrocytes. The extent to which neuronal glutamate transporters influence the lifetime of glutamate in the extracellular space remains unclear. Here we show that EAAC1, the predominant neuronal glutamate transporter at excitatory synapses in hippocampal area CA1, buffers glutamate released during synaptic events and prolongs the time course of its clearance by astrocytes. EAAC1 does not significantly alter activation of receptors in the synaptic cleft. Instead, it reduces recruitment of perisynaptic/extrasynaptic NR2B-containing NMDARs, thereby facilitating induction of long-term potentiation by short bursts of high-frequency stimulation. We describe novel roles of EAAC1 in regulating glutamate diffusion and propose that NMDARs at different subsynaptic locations can make distinct contributions to the regulation of synaptic strength.