@article {Sowa10282, author = {Nathaniel A. Sowa and Sarah E. Street and Pirkko Vihko and Mark J. Zylka}, title = {Prostatic Acid Phosphatase Reduces Thermal Sensitivity and Chronic Pain Sensitization by Depleting Phosphatidylinositol 4,5-Bisphosphate}, volume = {30}, number = {31}, pages = {10282--10293}, year = {2010}, doi = {10.1523/JNEUROSCI.2162-10.2010}, publisher = {Society for Neuroscience}, abstract = {Prostatic acid phosphatase (PAP) is expressed in nociceptive dorsal root ganglion (DRG) neurons, functions as an ectonucleotidase, and generates adenosine extracellularly. Here, we found that PAP inhibits noxious thermal sensitivity and sensitization that is associated with chronic pain through sustained activation of the adenosine A1 receptor (A1R) and phospholipase C-mediated depletion of phosphatidylinositol 4,5-bisphosphate (PIP2). In mice, intrathecal injection of PAP reduced PIP2 levels in DRGs, inhibited thermosensation through TRPV1, and enduringly reduced thermal hyperalgesia and mechanical allodynia caused by inflammation, nerve injury, and pronociceptive receptor activation. This included inhibitory effects on lysophosphatidic acid, purinergic (ATP), bradykinin, and protease-activated (thrombin) receptors. Conversely, PIP2 levels were significantly elevated in DRGs from Pap-/- mice, and this correlated with enhanced thermal hyperalgesia and mechanical allodynia in Pap-/- mice. To directly test the importance of PIP2 in nociception, we intrathecally injected PIP2 into mice. This transiently (2 h) elevated PIP2 levels in lumbar DRGs and transiently (2 h) enhanced thermosensation. Additionally, thermal hyperalgesia and mechanical allodynia were enduringly enhanced when PIP2 levels were elevated coincident with injury/pronociceptive receptor stimulation. Nociceptive sensitization was not affected if PIP2 levels were elevated in the absence of ongoing pronociceptive receptor stimulation. Together, our data suggest that PIP2 levels in DRGs directly influence thermosensation and the magnitude of nociceptive sensitization. Moreover, our data suggest there is an underlying {\textquotedblleft}phosphoinositide tone{\textquotedblright} that can be manipulated by an adenosine-generating ectonucleotidase. This tone regulates how effectively acute nociceptive insults promote the transition to chronic pain.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/30/31/10282}, eprint = {https://www.jneurosci.org/content/30/31/10282.full.pdf}, journal = {Journal of Neuroscience} }