TY - JOUR T1 - Hyperpolarization-Activated Currents in Gonadotropin-Releasing Hormone (GnRH) Neurons Contribute to Intrinsic Excitability and Are Regulated by Gonadal Steroid Feedback JF - The Journal of Neuroscience JO - J. Neurosci. SP - 13373 LP - 13383 DO - 10.1523/JNEUROSCI.1687-10.2010 VL - 30 IS - 40 AU - Zhiguo Chu AU - Hiroshi Takagi AU - Suzanne M. Moenter Y1 - 2010/10/06 UR - http://www.jneurosci.org/content/30/40/13373.abstract N2 - Pulsatile release of gonadotropin-releasing hormone (GnRH) is required for fertility and is regulated by steroid feedback. Hyperpolarization-activated currents (Ih) play a critical role in many rhythmic neurons. We examined the contribution of Ih to the membrane and firing properties of GnRH neurons and the modulation of this current by steroid milieu. Whole-cell voltage- and current-clamp recordings were made of GFP-identified GnRH neurons in brain slices from male mice that were gonad-intact, castrated, or castrated and treated with estradiol implants. APV, CNQX, and bicuculline were included to block fast synaptic transmission. GnRH neurons (47%) expressed a hyperpolarization-activated current with pharmacological and biophysical characteristics of Ih. The Ih-specific blocker ZD7288 reduced hyperpolarization-induced sag and rebound potential, decreased GnRH neuron excitability and action potential firing, and hyperpolarized membrane potential in some cells. ZD7288 also altered the pattern of burst firing and reduced the slope of recovery from the after-hyperpolarization potential. Activation of Ih by hyperpolarization increased spike frequency, whereas inactivation of Ih by depolarization reduced spike frequency. Castration increased Ih compared with that in gonad-intact males. This effect was reversed by in vivo estradiol replacement. Together, these data indicate Ih provides an excitatory drive in GnRH neurons that contributes to action potential burst firing and that estradiol regulates Ih in these cells. As estradiol is the primary central negative feedback hormone on GnRH neuron firing in males, this provides insight into the mechanisms by which steroid hormones potentially alter the intrinsic properties of GnRH neurons to change their activity. ER -