PT  - JOURNAL ARTICLE
AU  - Xiaowei Chen
AU  - Derek C. Molliver
AU  - G. F. Gebhart
TI  - The P2Y<sub>2</sub> Receptor Sensitizes Mouse Bladder Sensory Neurons and Facilitates Purinergic Currents
AID  - 10.1523/JNEUROSCI.5462-09.2010
DP  - 2010 Feb 10
TA  - The Journal of Neuroscience
PG  - 2365--2372
VI  - 30
IP  - 6
4099  - http://www.jneurosci.org/content/30/6/2365.short
4100  - http://www.jneurosci.org/content/30/6/2365.full
SO  - J. Neurosci.2010 Feb 10; 30
AB  - Sensitization of bladder afferents is an underlying contributor to the development and maintenance of painful bladder syndrome/interstitial cystitis. Extracellular purines and pyrimidines (e.g., ATP and UTP), released during bladder distension or from damaged cells after tissue insult, are thought to play an important role in bladder physiological and pathological states by actions at ionotropic P2X and metabotropic P2Y receptors. In the present study, we examined the ability of P2Y receptors to sensitize and modulate P2X-mediated responses in mouse bladder sensory neurons. UTP (a P2Y2 and P2Y4 agonist) increased excitability of bladder neurons by depolarizing resting membrane potential, increasing action potential firing, and facilitating responses to suprathreshold current injection as well as to P2X agonist application. These effects of UTP on bladder neuron excitability were blocked by the P2Y2 receptor antagonist suramin. UTP also facilitated bladder neuron homomeric P2X2 sustained currents and homomeric P2X3 fast currents. The facilitatory effect of UTP on P2X2 sustained currents was mediated by a G-protein-coupled P2Y2 receptor/PKC pathway, whereas the effect of UTP on P2X3 fast currents was G-protein independent. We also examined P2X and P2Y receptor expression in bladder neurons. P2Y2 and P2Y4 transcripts were detected in ∼50 and ∼20% of bladder neurons, respectively. Approximately 50% of P2X2- and P2X3-positive bladder neurons expressed P2Y2 transcripts, whereas ≤25% of the same bladder neurons expressed P2Y4 transcripts. These results support involvement of P2Y2 receptors in bladder sensation, suggesting an important contribution to bladder neuron excitability and hypersensitivity.