PT  - JOURNAL ARTICLE
AU  - Rogier Min
AU  - Guilherme Testa-Silva
AU  - Tim S. Heistek
AU  - Cathrin B. Canto
AU  - Johannes C. Lodder
AU  - Tiziana Bisogno
AU  - Vincenzo Di Marzo
AU  - Arjen B. Brussaard
AU  - Nail Burnashev
AU  - Huibert D. Mansvelder
TI  - Diacylglycerol Lipase Is Not Involved in Depolarization-Induced Suppression of Inhibition at Unitary Inhibitory Connections in Mouse Hippocampus
AID  - 10.1523/JNEUROSCI.BC-3622-09.2010
DP  - 2010 Feb 17
TA  - The Journal of Neuroscience
PG  - 2710--2715
VI  - 30
IP  - 7
4099  - http://www.jneurosci.org/content/30/7/2710.short
4100  - http://www.jneurosci.org/content/30/7/2710.full
SO  - J. Neurosci.2010 Feb 17; 30
AB  - Endocannabinoids control hippocampal inhibitory synaptic transmission through activation of presynaptic CB1 receptors. During depolarization-induced suppression of inhibition (DSI), endocannabinoids are synthesized upon postsynaptic depolarization. The endocannabinoid 2-arachidonoylglycerol (2-AG) may mediate hippocampal DSI. Currently, the best studied pathway for biosynthesis of 2-AG involves the enzyme diacylglycerol lipase (DAGL). However, whether DAGL is necessary for hippocampal DSI is controversial and was not systematically addressed. Here, we investigate DSI at unitary connections between CB1 receptor-containing interneurons and pyramidal neurons in CA1. We found that the novel DAGL inhibitor OMDM-188, as well as the established inhibitor RHC-80267, did not affect DSI. As reported previously, effects of the DAGL inhibitor tetrahydrolipstatin depended on the application method: postsynaptic intracellular application left DSI intact, while incubation blocked DSI. We show that all DAGL inhibitors tested block slow self-inhibition in neocortical interneurons, which involves DAGL. We conclude that DAGL is not involved in DSI at unitary connections in hippocampus.