RT Journal Article
SR Electronic
T1 Neurite regeneration by Aplysia neurons in dissociated cell culture: modulation by Aplysia hemolymph and the presence of the initial axonal segment
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2403
OP 2413
DO 10.1523/JNEUROSCI.03-12-02403.1983
VO 3
IS 12
A1 S Schacher
A1 E Proshansky
YR 1983
UL http://www.jneurosci.org/content/3/12/2403.abstract
AB Neurons from the abdominal ganglion of the mollusc Aplysia californica regenerate neurite processes in dissociated cell culture. Both the nature of neurite outgrowth and the morphology of the cells are influenced by the presence of adult Aplysia hemolymph in the growth medium and the presence of a portion of a cell's original axonal process. Aplysia hemolymph enhances cell survival, the initiation of neurite outgrowth from multiple sites on the cell body surface, the linear growth of the processes, and the amount of branching by those processes. Hemolymph also decreases the diameter of the outgrowing neurite fascicles and the diameter of the individual neurites within the fascicles. The presence of a cell's original axon reduces the time required for the initiation of neurite outgrowth and restricts the formation of multipolar processes. In addition, the presence of an initial axonal segment is essential for neurite regeneration from large adult neurons.