RT Journal Article
SR Electronic
T1 Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 376
OP 382
DO 10.1523/JNEUROSCI.03-02-00376.1983
VO 3
IS 2
A1 Neale, EA
A1 Oertel, WH
A1 Bowers, LM
A1 Weise, VK
YR 1983
UL http://www.jneurosci.org/content/3/2/376.abstract
AB In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake.