RT Journal Article
SR Electronic
T1 Quantitative autoradiographic distribution of L-[3H]glutamate-binding sites in rat central nervous system
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2133
OP 2144
DO 10.1523/JNEUROSCI.04-08-02133.1984
VO 4
IS 8
A1 JT Greenamyre
A1 AB Young
A1 JB Penney
YR 1984
UL http://www.jneurosci.org/content/4/8/2133.abstract
AB Quantitative autoradiography was used to determine the distribution of L-[3H]glutamate-binding sites in the rat central nervous system. Autoradiography was carried out in the presence of Cl- and Ca2+ ions. Scatchard plots and Hill coefficients of glutamate binding suggested that glutamate was interacting with a single population of sites having a K-D of about 300 nM and a capacity of 14.5 pmol/mg of protein. In displacement studies, ibotenate also appeared to bind to a single class of non-interacting sites with a KI of 28 microM. However, quisqualate displacement of [3H]glutamate binding revealed two well-resolved sites with KIS of 12 nM and 114 microM in striatum. These sites were unevenly distributed, representing different proportions of specific glutamate binding in different brain regions. The distribution of glutamate- binding sites correlated very well with the projection areas of putative glutamatergic pathways. This technique provides an extremely sensitive assay which can be used to gather detailed pharmacological and anatomical information about L-[3H]glutamate binding in the central nervous system.