PT - JOURNAL ARTICLE AU - Walter, MF AU - Kiger, JA TI - The Dunce gene of Drosophila: roles of Ca2+ and calmodulin in adenosine 3':5'-cyclic monophosphate-specific phosphodiesterase activity AID - 10.1523/JNEUROSCI.04-02-00495.1984 DP - 1984 Feb 01 TA - The Journal of Neuroscience PG - 495--501 VI - 4 IP - 2 4099 - http://www.jneurosci.org/content/4/2/495.short 4100 - http://www.jneurosci.org/content/4/2/495.full SO - J. Neurosci.1984 Feb 01; 4 AB - Two genetically distinct forms of cyclic nucleotide phosphodiesterases are present in adult Drosophila melanogaster. Form II, which specifically hydrolyzes adenosine 3′:5′-cyclic monophosphate (cAMP), is controlled by the dunce+ gene. Mutants of this gene either eliminate this enzyme form entirely or alter its kinetic and thermal properties, suggesting that dunce+ is the structural gene for this enzyme. These mutants are defective in memory formation, habituation, and sensitization and exhibit elevated cAMP levels, implicating cAMP in these neurological processes. The other phosphodiesterase, Form I, which hydrolyzes both cAMP and guanosine 3′:5′-cyclic monophosphate (cGMP), is not affected by dunce mutations. Because both cAMP and Ca2+ serve as intracellular second messengers in mediating the effects of neurotransmitters, the effects of Ca2+ on each form of phosphodiesterase have been investigated. Previous work has suggested that Form I is activated by calmodulin in a Ca2+-dependent manner. We confirm this activation and demonstrate that the activation involves the Ca2+-dependent association of two molecules of calmodulin with one Form I molecule. Under conditions permitting activation and association of Form I with calmodulin, we observe no interaction of Ca2+/calmodulin with Form II. Our studies suggest that the primary physiological defect, associated with a defective or absent Form II cAMP-specific phosphodiesterase and leading to the dunce neurological phenotype, is due to a direct failure to regulate the cAMP level in nerve cells rather than to a failure to mediate a signal resulting from a cAMP- induced Ca2+ influx, associated with presynaptic facilitation.