PT - JOURNAL ARTICLE AU - O'Shea, M AU - Witten, J AU - Schaffer, M TI - Isolation and characterization of two myoactive neuropeptides: further evidence of an invertebrate peptide family AID - 10.1523/JNEUROSCI.04-02-00521.1984 DP - 1984 Feb 01 TA - The Journal of Neuroscience PG - 521--529 VI - 4 IP - 2 4099 - http://www.jneurosci.org/content/4/2/521.short 4100 - http://www.jneurosci.org/content/4/2/521.full SO - J. Neurosci.1984 Feb 01; 4 AB - The neuropeptide proctolin acts as a neuromuscular co-transmitter in insect skeletal muscle. As a prelude to determining whether other peptides may function in a similar way, we are attempting to isolate and characterize the chemical nature of new myoactive neuropeptides in insects. We examined the corpus cardiacum, a major insect neurosecretory structure of the American cockroach (Periplaneta americana), using a skeletal muscle bioassay and high pressure liquid chromatography fractionation and identified two myoactive factors, MI and MII. They are synthesized in the corpus cardiacum and released from it into the blood by a calcium-dependent mechanism. Amino acid and fast atom bombardment-mass spectroscopy analysis show that MI and MII are structurally related octapeptides representing the major secreted products of the cockroach corpus cardiacum. Both MI and MII are also present in the CNS and in the gut, indicating transmitter as well as hormonal functions in the cockroach. A survey in other species indicates MI may be present in invertebrates other than insects, but neither was found in the rat. The MI and MII peptides have clear chemical affinities to two previously described invertebrate peptides, locust adipokinetic hormone and crustacean red pigment concentrating hormone, as well as sharing biological activity with these peptides. Our results provide further evidence for the existence of a large family of structurally related peptides with divergent functions in a variety of invertebrate types.