PT - JOURNAL ARTICLE AU - JR Holtman, Jr AU - TE Dick AU - AJ Berger TI - Involvement of serotonin in the excitation of phrenic motoneurons evoked by stimulation of the raphe obscurus AID - 10.1523/JNEUROSCI.06-04-01185.1986 DP - 1986 Apr 01 TA - The Journal of Neuroscience PG - 1185--1193 VI - 6 IP - 4 4099 - http://www.jneurosci.org/content/6/4/1185.short 4100 - http://www.jneurosci.org/content/6/4/1185.full SO - J. Neurosci.1986 Apr 01; 6 AB - Short-latency averaged responses in the C5 phrenic nerves to electrical stimulation (2.5–80 microA; 5–80 Hz; 150 microseconds pulse duration) of raphe pallidus (RP) and raphe obscurus (RO) were investigated in anesthetized, paralyzed, and artificially ventilated cats. The responses to stimulation of RO were excitatory, whereas a mixture of inhibitory and excitatory responses of lesser magnitude were observed after stimulating in RP. The maximal response was obtained from the ventral part of RO and consisted of early and delayed excitatory responses that were of equal magnitude in both left and right C5 phrenic nerve roots. The mean latency for the early response was 2.5 +/- 0.1 msec and for the delayed response was 7.0 +/- 0.2 msec. Both responses were elicited during inspiratory phase stimulation, but only the delayed response was present during expiratory phase stimulation. The stimulus threshold of the early response was 5 microA; the delayed response was elicited at currents as small as 2.5 microA. Early and delayed responses were affected in different ways by increasing stimulus current and by increasing stimulus frequency. Intravenous administration of serotonin receptor antagonists methysergide (0.1–0.7 mg/kg), metergoline (33–244 micrograms/kg), and cinanserin (1.5–9.0 micrograms/kg) caused significant dose-related reductions in the magnitude of the delayed response, but did not significantly affect the early response. These data suggest that the early and delayed excitatory responses are mediated by different neuronal pathways. The early response does not involve serotonin release, while the later response is mediated at least in part by activation of a serotonergic pathway.(ABSTRACT TRUNCATED AT 250 WORDS)