TY - JOUR T1 - Fluorescence-activated cell sorting of embryonic mouse and rat motoneurons and their long-term survival in vitro JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3088 LP - 3104 DO - 10.1523/JNEUROSCI.07-10-03088.1987 VL - 7 IS - 10 AU - AE Schaffner AU - PA St. John AU - JL Barker Y1 - 1987/10/01 UR - http://www.jneurosci.org/content/7/10/3088.abstract N2 - Motoneurons from embryonic mice and rats were labeled with retrogradely transported succinyl wheat germ agglutinin (WGA)-fluorescein isothiocyanate (FITC). After dissociation of the spinal cord, fluorescent motoneurons were isolated by flow cytometry. Sorted motoneurons were maintained for as long as 6 weeks in vitro on monolayers of astrocytes in muscle-conditioned medium. Immunocytochemical staining of the cultures for various neuronal antigens suggested that sorted motoneurons are receptive to GABAergic and glycinergic, as well as cholinergic, innervation. Many of the sorted cells were also labeled intracellularly with antibodies to choline acetyltransferase (ChAT) and GABA. ER -