PT  - JOURNAL ARTICLE
AU  - RG Pourcho
AU  - DJ Goebel
TI  - A combined Golgi and autoradiographic study of 3H-glycine-accumulating cone bipolar cells in the cat retina
AID  - 10.1523/JNEUROSCI.07-04-01178.1987
DP  - 1987 Apr 01
TA  - The Journal of Neuroscience
PG  - 1178--1188
VI  - 7
IP  - 4
4099  - http://www.jneurosci.org/content/7/4/1178.short
4100  - http://www.jneurosci.org/content/7/4/1178.full
SO  - J. Neurosci.1987 Apr 01; 7
AB  - Morphological subclasses of cone bipolar cells in the cat retina were studied in Golgi preparations and through the combination of Golgi impregnation with quantitative electron-microscopic autoradiography of 3H-glycine accumulation. Camera lucida drawings, computer-assisted rotations, and radial sections provided the morphological details used in assigning individual cells to morphologically defined subclasses. Selected Golgi-impregnated cells from retinas preloaded with 3H-glycine were sectioned for autoradiographic analysis. Grain densities were quantitated and compared on a normalized scale with the labeling of amacrine cells with 3H-glycine. Eleven types of cone bipolar cells were identified, 6 with axon terminals ramifying in sublamina a and 5 with axons terminating in sublamina b. Of these cells, types CBb2 and CBb5 showed the highest affinity for glycine, with labeling at 41 and 38% with respect to the most heavily labeled amacrine cells. Types CBb1, CBb3, CBa2, and CBa6 were labeled at approximately 20% of maximal labeling. The accumulation of glycine by these cells suggests that they may be involved in providing inhibitory input to the inner retina.