RT Journal Article
SR Electronic
T1 Morphological and immunocytochemical identification of indoleamine- accumulating neurons in the cat retina
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 1574
OP 1585
DO 10.1523/JNEUROSCI.07-05-01574.1987
VO 7
IS 5
A1 Wassle, H
A1 Voigt, T
A1 Patel, B
YR 1987
UL http://www.jneurosci.org/content/7/5/1574.abstract
AB The type and distribution of indoleamine-accumulating neurons were studied in the cat retina. The neurons were characterized by their capacity to take up an exogenous indoleamine, 5,6-dihydroxytryptamine, which could then be visualized using formaldehyde-induced fluorescence. Under direct microscopic control, intracellular injection of Lucifer yellow into the labeled cells revealed that the indoleamine- accumulating neurons comprise 2 distinct morphological types of amacrine cell and 1 class of ganglion cell. The dendritic morphology and retinal distribution of these cells were studied in detail. The proportion of retinal cells that accumulate 5-HT was measured using 5- HT (5-hydroxytryptamine) preloading in vitro and immunocytochemistry with an anti-5-HT antibody. Only 1 type of amacrine cell showed uptake of 5-HT at a concentration of 10(-7) M. Cells containing endogenous 5- HT were not detected by immunocytochemistry.