RT Journal Article
SR Electronic
T1 Excitatory amino acid receptors coupled with guanylate cyclase in primary cultures of cerebellar granule cells
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 40
OP 47
DO 10.1523/JNEUROSCI.07-01-00040.1987
VO 7
IS 1
A1 Novelli, A
A1 Nicoletti, F
A1 Wroblewski, JT
A1 Alho, H
A1 Costa, E
A1 Guidotti, A
YR 1987
UL http://www.jneurosci.org/content/7/1/40.abstract
AB Primary cultures of cerebellar granule cells have been used in pharmacologically and functionally characterizing excitatory amino acid recognition sites coupled with guanylate cyclase. When granule cells were incubated in physiological culture conditions (Locke's solution, pH 7.4), only kainate and, to a lesser extent, L-glutamate increased cyclic GMP (cGMP) levels. Under these conditions, L-aspartate, N-methyl- D-aspartate (NMDA), and quisqualate were inactive. When granule cells were incubated in the absence of extracellular Mg2+ or in the presence of the depolarizing agent veratrine, L-glutamate, L-aspartate, and NMDA became as effective as kainate in enhancing cGMP formation. The action of kainate was preferentially antagonized by 2,3-cis- piperidindicarboxylate, whereas the action of L-glutamate was preferentially antagonized by (+/-)2-amino-5-phosphonovalerate. These data suggest that 2 different excitatory amino acid recognition sites (activated by kainate or by L-glutamate, L-aspartate, and NMDA, respectively) are coupled with guanylate cyclase in primary cultures of cerebellar granule cells: While the coupling of the recognition site for kainate with guanylate cyclase operates under resting conditions and in the presence of Mg2+, the coupling of the recognition site for L- glutamate, L-aspartate, and NMDA with guanylate cyclase requires depolarizing conditions or the absence of extracellular Mg2+.