TY - JOUR T1 - Progranulin Deficiency Decreases Gross Neural Connectivity But Enhances Transmission at Individual Synapses JF - The Journal of Neuroscience JO - J. Neurosci. SP - 11126 LP - 11132 DO - 10.1523/JNEUROSCI.6244-10.2011 VL - 31 IS - 31 AU - Lucia Tapia AU - Austen Milnerwood AU - Aobo Guo AU - Fergil Mills AU - Eileen Yoshida AU - Cristina Vasuta AU - Ian R. Mackenzie AU - Lynn Raymond AU - Max Cynader AU - William Jia AU - Shernaz X. Bamji Y1 - 2011/08/03 UR - http://www.jneurosci.org/content/31/31/11126.abstract N2 - Frontotemporal dementia (FTD) has been linked to mutations in the progranulin gene (GRN) that lead to progranulin (PGRN) haploinsufficiency. Thus far, our understanding of the effects of PGRN depletion in the brain has been derived from investigation of gross pathology, and more detailed analyses of cellular function have been lacking. We report that knocking down PGRN levels in rat primary hippocampal cultures reduces neural connectivity by decreasing neuronal arborization and length as well as synapse density. Despite this, the number of synaptic vesicles per synapse and the frequency of mEPSCs are increased in PGRN knockdown cells, suggesting an increase in the probability of release at remaining synapses. Interestingly, we demonstrate that the number of vesicles per synapse is also increased in postmortem brain sections from FTD patients with PGRN haploinsufficiency, relative to controls. Our observations show that PGRN knockdown severely alters neuronal connectivity in vitro and that the synaptic vesicle phenotype observed in culture is consistent with that observed in the hippocampus of FTD patients. ER -