RT Journal Article
SR Electronic
T1 Enhanced Sucrose and Cocaine Self-Administration and Cue-Induced Drug Seeking after Loss of VGLUT2 in Midbrain Dopamine Neurons in Mice
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 12593
OP 12603
DO 10.1523/JNEUROSCI.2397-11.2011
VO 31
IS 35
A1 Alsiö, Johan
A1 Nordenankar, Karin
A1 Arvidsson, Emma
A1 Birgner, Carolina
A1 Mahmoudi, Souha
A1 Halbout, Briac
A1 Smith, Casey
A1 Fortin, Guillaume M.
A1 Olson, Lars
A1 Descarries, Laurent
A1 Trudeau, Louis-Éric
A1 Kullander, Klas
A1 Lévesque, Daniel
A1 Wallén-Mackenzie, Åsa
YR 2011
UL http://www.jneurosci.org/content/31/35/12593.abstract
AB The mesostriatal dopamine (DA) system contributes to several aspects of responses to rewarding substances and is implicated in conditions such as drug addiction and eating disorders. A subset of DA neurons has been shown to express the type 2 Vesicular glutamate transporter (Vglut2) and may therefore corelease glutamate. In the present study, we analyzed mice with a conditional deletion of Vglut2 in DA neurons (Vglut2f/f;DAT-Cre) to address the functional significance of the glutamate–DA cophenotype for responses to cocaine and food reinforcement. Biochemical parameters of striatal DA function were also examined by using DA receptor autoradiography, immediate-early gene quantitative in situ hybridization after cocaine challenge, and DA-selective in vivo chronoamperometry. Mice in which Vglut2 expression had been abrogated in DA neurons displayed enhanced operant self-administration of both high-sucrose food and intravenous cocaine. Furthermore, cocaine seeking maintained by drug-paired cues was increased by 76%, showing that reward-dependent plasticity is perturbed in these mice. In addition, several lines of evidence suggest that adaptive changes occurred in both the ventral and dorsal striatum in the absence of VGLUT2: DA receptor binding was increased, and basal mRNA levels of the DA-induced early genes Nur77 and c-fos were elevated as after cocaine induction. Furthermore, in vivo challenge of the DA system by potassium-evoked depolarization revealed less DA release in both striatal areas. This study demonstrates that absence of VGLUT2 in DA neurons leads to perturbations of reward consumption as well as reward-associated memory, features of particular relevance for addictive-like behavior.