PT  - JOURNAL ARTICLE
AU  - Ramanantsoa, Nelina
AU  - Hirsch, Marie-Rose
AU  - Thoby-Brisson, Muriel
AU  - Dubreuil, Véronique
AU  - Bouvier, Julien
AU  - Ruffault, Pierre-Louis
AU  - Matrot, Boris
AU  - Fortin, Gilles
AU  - Brunet, Jean-François
AU  - Gallego, Jorge
AU  - Goridis, Christo
TI  - Breathing without CO<sub>2</sub> Chemosensitivity in Conditional <em>Phox2b</em> Mutants
AID  - 10.1523/JNEUROSCI.1721-11.2011
DP  - 2011 Sep 07
TA  - The Journal of Neuroscience
PG  - 12880--12888
VI  - 31
IP  - 36
4099  - http://www.jneurosci.org/content/31/36/12880.short
4100  - http://www.jneurosci.org/content/31/36/12880.full
SO  - J. Neurosci.2011 Sep 07; 31
AB  - Breathing is a spontaneous, rhythmic motor behavior critical for maintaining O2, CO2, and pH homeostasis. In mammals, it is generated by a neuronal network in the lower brainstem, the respiratory rhythm generator (Feldman et al., 2003). A century-old tenet in respiratory physiology posits that the respiratory chemoreflex, the stimulation of breathing by an increase in partial pressure of CO2 in the blood, is indispensable for rhythmic breathing. Here we have revisited this postulate with the help of mouse genetics. We have engineered a conditional mouse mutant in which the toxic PHOX2B27Ala mutation that causes congenital central hypoventilation syndrome in man is targeted to the retrotrapezoid nucleus, a site essential for central chemosensitivity. The mutants lack a retrotrapezoid nucleus and their breathing is not stimulated by elevated CO2 at least up to postnatal day 9 and they barely respond as juveniles, but nevertheless survive, breathe normally beyond the first days after birth, and maintain blood PCO2 within the normal range. Input from peripheral chemoreceptors that sense PO2 in the blood appears to compensate for the missing CO2 response since silencing them by high O2 abolishes rhythmic breathing. CO2 chemosensitivity partially recovered in adulthood. Hence, during the early life of rodents, the excitatory input normally afforded by elevated CO2 is dispensable for life-sustaining breathing and maintaining CO2 homeostasis in the blood.