PT  - JOURNAL ARTICLE
AU  - Tao Ma
AU  - Nikos Tzavaras
AU  - Panayiotis Tsokas
AU  - Emmanuel M. Landau
AU  - Robert D. Blitzer
TI  - Synaptic Stimulation of mTOR Is Mediated by Wnt Signaling and Regulation of Glycogen Synthetase Kinase-3
AID  - 10.1523/JNEUROSCI.4761-11.2011
DP  - 2011 Nov 30
TA  - The Journal of Neuroscience
PG  - 17537--17546
VI  - 31
IP  - 48
4099  - http://www.jneurosci.org/content/31/48/17537.short
4100  - http://www.jneurosci.org/content/31/48/17537.full
SO  - J. Neurosci.2011 Nov 30; 31
AB  - The persistent or “late” phase of long-term potentiation (L-LTP), which requires protein synthesis, can be induced by relatively intense synaptic activity. The ability of such strong synaptic protocols to engage the translational machinery and produce plasticity-related proteins, while weaker protocols activate only posttranslational processes and transient potentiation (early LTP; E-LTP), is not understood. Among the major translation control pathways in neurons, the stimulation of mammalian target of rapamycin (mTOR) is a key event in the induction of L-LTP. We report that mTOR is tonically suppressed in rat hippocampus under resting conditions, a consequence of the basal activity of glycogen synthetase kinase 3 (GSK3). This suppression could be overcome by weak synaptic stimulation in the presence of the β-adrenergic agonist isoproterenol, a combination that induced L-LTP, and activation of mTOR coincided with the Akt-mediated phosphorylation of GSK3. Surprisingly, while isoproterenol alone elevated Akt activity, it failed to increase GSK3 phosphorylation or mTOR signaling, showing that Akt was uncoupled from these effectors in the absence of synaptic stimulation. With the addition of weak stimulation, Akt signaled to GSK3 and mTOR, a gating effect that was mediated by voltage-dependent Ca2+ channels and the Wnt pathway. mTOR could be stimulated by pharmacological inhibition, enabling weak HFS to induce L-LTP. These results establish GSK3 as an integrator of Akt and Wnt signals and suggest that overcoming GSK3-mediated suppression of mTOR is a key event in the induction of L-LTP by synaptic activity.