PT  - JOURNAL ARTICLE
AU  - Li-Hua Yao
AU  - Yan Rao
AU  - Kelly Varga
AU  - Chun-Yang Wang
AU  - Peng Xiao
AU  - Manfred Lindau
AU  - Liang-Wei Gong
TI  - Synaptotagmin 1 Is Necessary for the Ca<sup>2+</sup> Dependence of Clathrin-Mediated Endocytosis
AID  - 10.1523/JNEUROSCI.3540-11.2012
DP  - 2012 Mar 14
TA  - The Journal of Neuroscience
PG  - 3778--3785
VI  - 32
IP  - 11
4099  - http://www.jneurosci.org/content/32/11/3778.short
4100  - http://www.jneurosci.org/content/32/11/3778.full
SO  - J. Neurosci.2012 Mar 14; 32
AB  - The role of Ca2+ in synaptic vesicle endocytosis remains uncertain due to the diversity in various preparations where several forms of endocytosis may contribute variably in different conditions. Although recent studies have demonstrated that Ca2+ is important for clathrin-mediated endocytosis (CME), the mechanistic role of Ca2+ in CME remains to be elucidated. By monitoring CME of single vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond time resolution, we demonstrate that the dynamics of vesicle fission during CME is Ca2+ dependent but becomes Ca2+ independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca2+ dependence of CME.