RT Journal Article
SR Electronic
T1 Synaptotagmin 1 Is Necessary for the Ca<sup>2+</sup> Dependence of Clathrin-Mediated Endocytosis
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 3778
OP 3785
DO 10.1523/JNEUROSCI.3540-11.2012
VO 32
IS 11
A1 Li-Hua Yao
A1 Yan Rao
A1 Kelly Varga
A1 Chun-Yang Wang
A1 Peng Xiao
A1 Manfred Lindau
A1 Liang-Wei Gong
YR 2012
UL http://www.jneurosci.org/content/32/11/3778.abstract
AB The role of Ca2+ in synaptic vesicle endocytosis remains uncertain due to the diversity in various preparations where several forms of endocytosis may contribute variably in different conditions. Although recent studies have demonstrated that Ca2+ is important for clathrin-mediated endocytosis (CME), the mechanistic role of Ca2+ in CME remains to be elucidated. By monitoring CME of single vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond time resolution, we demonstrate that the dynamics of vesicle fission during CME is Ca2+ dependent but becomes Ca2+ independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca2+ dependence of CME.