RT Journal Article
SR Electronic
T1 Neuronal Classification and Marker Gene Identification via Single-Cell Expression Profiling of Brainstem Vestibular Neurons Subserving Cerebellar Learning
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7819
OP 7831
DO 10.1523/JNEUROSCI.0543-12.2012
VO 32
IS 23
A1 Takashi Kodama
A1 Shiloh Guerrero
A1 Minyoung Shin
A1 Seti Moghadam
A1 Michael Faulstich
A1 Sascha du Lac
YR 2012
UL http://www.jneurosci.org/content/32/23/7819.abstract
AB Identification of marker genes expressed in specific cell types is essential for the genetic dissection of neural circuits. Here we report a new strategy for classifying heterogeneous populations of neurons into functionally distinct types and for identifying associated marker genes. Quantitative single-cell expression profiling of genes related to neurotransmitters and ion channels enables functional classification of neurons; transcript profiles for marker gene candidates identify molecular handles for manipulating each cell type. We apply this strategy to the mouse medial vestibular nucleus (MVN), which comprises several types of neurons subserving cerebellar-dependent learning in the vestibulo-ocular reflex. Ion channel gene expression differed both qualitatively and quantitatively across cell types and could distinguish subtle differences in intrinsic electrophysiology. Single-cell transcript profiling of MVN neurons established six functionally distinct cell types and associated marker genes. This strategy is applicable throughout the nervous system and could facilitate the use of molecular genetic tools to examine the behavioral roles of distinct neuronal populations.