TY - JOUR T1 - GABAergic Inhibition of Histaminergic Neurons Regulates Active Waking But Not the Sleep–Wake Switch or Propofol-Induced Loss of Consciousness JF - The Journal of Neuroscience JO - J. Neurosci. SP - 13062 LP - 13075 DO - 10.1523/JNEUROSCI.2931-12.2012 VL - 32 IS - 38 AU - Anna Y. Zecharia AU - Xiao Yu AU - Thomas Götz AU - Zhiwen Ye AU - David R. Carr AU - Peer Wulff AU - Bernhard Bettler AU - Alexei L. Vyssotski AU - Stephen G. Brickley AU - Nicholas P. Franks AU - William Wisden Y1 - 2012/09/19 UR - http://www.jneurosci.org/content/32/38/13062.abstract N2 - The activity of histaminergic neurons in the tuberomammillary nucleus (TMN) of the hypothalamus correlates with an animal's behavioral state and maintains arousal. We examined how GABAergic inputs onto histaminergic neurons regulate this behavior. A prominent hypothesis, the “flip-flop” model, predicts that increased and sustained GABAergic drive onto these cells promotes sleep. Similarly, because of the histaminergic neurons' key hub-like place in the arousal circuitry, it has also been suggested that anesthetics such as propofol induce loss of consciousness by acting primarily at histaminergic neurons. We tested both these hypotheses in mice by genetically removing ionotropic GABAA or metabotropic GABAB receptors from histidine decarboxylase-expressing neurons. At the cellular level, histaminergic neurons deficient in synaptic GABAA receptors were significantly more excitable and were insensitive to the anesthetic propofol. At the behavioral level, EEG profiles were recorded in nontethered mice over 24 h. Surprisingly, GABAergic transmission onto histaminergic neurons had no effect in regulating the natural sleep–wake cycle and, in the case of GABAA receptors, for propofol-induced loss of righting reflex. The latter finding makes it unlikely that the histaminergic TMN has a central role in anesthesia. GABAB receptors on histaminergic neurons were dispensable for all behaviors examined. Synaptic inhibition of histaminergic cells by GABAA receptors, however, was essential for habituation to a novel environment. ER -