TY - JOUR T1 - KAYAK-α Modulates Circadian Transcriptional Feedback Loops in <em>Drosophila</em> Pacemaker Neurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 16959 LP - 16970 DO - 10.1523/JNEUROSCI.1888-12.2012 VL - 32 IS - 47 AU - Jinli Ling AU - Raphaëlle Dubruille AU - Patrick Emery Y1 - 2012/11/21 UR - http://www.jneurosci.org/content/32/47/16959.abstract N2 - Circadian rhythms are generated by well-conserved interlocked transcriptional feedback loops in animals. In Drosophila, the dimeric transcription factor CLOCK/CYCLE (CLK/CYC) promotes period (per), timeless (tim), vrille (vri), and PAR-domain protein 1 (Pdp1) transcription. PER and TIM negatively feed back on CLK/CYC transcriptional activity, whereas VRI and PDP1 negatively and positively regulate Clk transcription, respectively. Here, we show that the α isoform of the Drosophila FOS homolog KAYAK (KAY) is required for normal circadian behavior. KAY-α downregulation in circadian pacemaker neurons increases period length by 1.5 h. This behavioral phenotype is correlated with decreased expression of several circadian proteins. The strongest effects are on CLK and the neuropeptide PIGMENT DISPERSING FACTOR, which are both under VRI and PDP1 control. Consistently, KAY-α can bind to VRI and inhibit its interaction with the Clk promoter. Interestingly, KAY-α can also repress CLK activity. Hence, in flies with low KAY-α levels, CLK derepression would partially compensate for increased VRI repression, thus attenuating the consequences of KAY-α downregulation on CLK targets. We propose that the double role of KAY-α in the two transcriptional loops controlling Drosophila circadian behavior brings precision and stability to their oscillations. ER -