RT Journal Article
SR Electronic
T1 Dynamic and Permissive Roles of TRPV1 and TRPV4 Channels for Thermosensation in Mouse Supraoptic Magnocellular Neurosecretory Neurons
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 17160
OP 17165
DO 10.1523/JNEUROSCI.1048-13.2013
VO 33
IS 43
A1 Jessica R. Sudbury
A1 Charles W. Bourque
YR 2013
UL http://www.jneurosci.org/content/33/43/17160.abstract
AB The transient receptor potential vanilloid 1 and 4 genes (trpv1, trpv4) encode temperature-sensitive cation channels hypothesized to mediate thermoresponses in mammalian cells. Although such channels were shown to participate in the peripheral detection of ambient temperature, the specific roles of these channels in central thermosensory neurons remain unclear. Here we report that the membrane potential and excitability of mouse magnocellular neurosecretory cells (MNCs) maintained at physiological temperature were lowered in an additive manner upon pharmacological blockade, or genetic deletion, of trpv1 and trpv4. However extracellular recordings from spontaneously active MNCs in situ showed that blockade or genetic deletion of trpv4 does not interfere with thermally induced changes in action potential firing, whereas loss of trpv1 abolished this phenotype. These findings indicate that channels encoded by trpv4 play a permissive role that contributes to basal electrical activity, but that trpv1 plays a dynamic role that is required for physiological thermosensation by MNCs.