RT Journal Article SR Electronic T1 Stress Switches Cannabinoid Type-1 (CB1) Receptor-Dependent Plasticity from LTD to LTP in the Bed Nucleus of the Stria Terminalis JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 19657 OP 19663 DO 10.1523/JNEUROSCI.3175-13.2013 VO 33 IS 50 A1 Glangetas, Christelle A1 Girard, Delphine A1 Groc, Laurent A1 Marsicano, Giovanni A1 Chaouloff, Francis A1 Georges, François YR 2013 UL http://www.jneurosci.org/content/33/50/19657.abstract AB The bed nucleus of the stria terminalis (BNST) exerts a coordinated modulation of the psychoneuroendocrine responses to stress. However, how acute stress impacts on BNST in vivo plasticity is a crucial question that still remains unanswered. Here, neurons from the anterior portion of the BNST (aBNST) were recorded in vivo during and after stimulation of their medial prefrontal cortical (mPFC) afferents. In C57BL/6N mice, a 1 h restraint stress induced a switch from long-term depression (LTD) to long-term potentiation (LTP) in the aBNST after a 10 Hz mPFC stimulation. This switch was independent from glucocorticoid receptor stimulation. Because the endocannabinoid system regulates aBNST activity, we next examined the role of cannabinoid type-1 receptors (CB1-Rs) in these changes. Mutant mice lacking CB1-Rs (CB1−/− mice) displayed a marked deficit in the ability to develop plasticity under control and stress conditions, compared with their wild-type littermates (CB1+/+ mice). This difference was not accounted for by genetic differences in stress sensitivity, as revealed by Fos immunohistochemistry analyses. Local blockade of CB1-Rs in the aBNST and the use of mutant mice bearing a selective deletion of CB1-Rs in cortical glutamatergic neurons indicated that stress-elicited LTP involved CB1-Rs located on aBNST excitatory terminals. These results show that acute stress reverts LTD into LTP in the aBNST and that the endocannabinoid system plays a key role therein.