RT Journal Article SR Electronic T1 Astroglial-Derived Periostin Promotes Axonal Regeneration after Spinal Cord Injury JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 2438 OP 2443 DO 10.1523/JNEUROSCI.2947-13.2014 VO 34 IS 7 A1 Chung-Hsuan Shih A1 Michelle Lacagnina A1 Kelly Leuer-Bisciotti A1 Christoph Pröschel YR 2014 UL http://www.jneurosci.org/content/34/7/2438.abstract AB Traumatic spinal cord injury (SCI) results in a cascade of tissue responses leading to cell death, axonal degeneration, and glial scar formation, exacerbating the already hostile environment and further inhibiting axon regeneration. Overcoming these inhibitory cues and promoting axonal regeneration is one of the primary targets in developing a cure for SCI. Previously, we demonstrated that transplantation of bone morphogenetic protein (BMP)-induced astrocytes derived from embryonic glial-restricted precursors (GDAsBMP) promotes extensive axonal growth and motor function recovery in a rodent spinal cord injury model. Here, we identify periostin (POSTN), a secreted protein, as a key component of GDABMP-induced axonal regeneration. POSTN is highly expressed by GDAsBMP and the perturbation of POSTN expression by shRNA diminished GDABMP-induced neurite extension in vitro. We also found that recombinant POSTN is sufficient to overcome the inhibitory effect of scar-associated molecules and promote neurite extension in vitro by signaling through focal adhesion kinase and Akt. Furthermore, transplantation of POSTN-deficient GDAsBMP into the injured rat spinal cord resulted in compromised axonal regeneration, indicating that POSTN plays an essential role in GDABMP-mediated axonal regeneration. This finding reveals not only one of the major mechanisms underlying GDABMP-dependent recovery from SCI, but also the potential of POSTN as a therapeutic agent for traumatic injury of the CNS.