RT Journal Article SR Electronic T1 The TRIM-NHL Protein Brat Promotes Axon Maintenance by Repressing src64B Expression JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 13855 OP 13864 DO 10.1523/JNEUROSCI.3285-13.2014 VO 34 IS 41 A1 Giovanni Marchetti A1 Ilka Reichardt A1 Juergen A. Knoblich A1 Florence Besse YR 2014 UL http://www.jneurosci.org/content/34/41/13855.abstract AB The morphology and the connectivity of neuronal structures formed during early development must be actively maintained as the brain matures. Although impaired axon stability is associated with the progression of various neurological diseases, relatively little is known about the factors controlling this process. We identified Brain tumor (Brat), a conserved member of the TRIM-NHL family of proteins, as a new regulator of axon maintenance in Drosophila CNS. Brat function is dispensable for the initial growth of Mushroom Body axons, but is required for the stabilization of axon bundles. We found that Brat represses the translation of src64B, an upstream regulator of a conserved Rho-dependent pathway previously shown to promote axon retraction. Furthermore, brat phenotypes are phenocopied by src64B overexpression, and partially suppressed by reducing the levels of src64B or components of the Rho pathway, suggesting that brat promotes axon maintenance by downregulating the levels of Src64B. Finally, Brat regulates brain connectivity via its NHL domain, but independently of its previously described partners Nanos, Pumilio, and d4EHP. Thus, our results uncover a novel post-transcriptional regulatory mechanism that controls the maintenance of neuronal architecture by tuning the levels of a conserved rho-dependent signaling pathway.