RT Journal Article
SR Electronic
T1 Coassembly and Coupling of SK2 Channels and mGlu<sub>5</sub> Receptors
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 14793
OP 14802
DO 10.1523/JNEUROSCI.2038-14.2014
VO 34
IS 44
A1 Gloria García-Negredo
A1 David Soto
A1 Javier Llorente
A1 Xavier Morató
A1 Koen M.O. Galenkamp
A1 Maricel Gómez-Soler
A1 Víctor Fernández-Dueñas
A1 Masahiko Watanabe
A1 John P. Adelman
A1 Ryuichi Shigemoto
A1 Yugo Fukazawa
A1 Rafael Luján
A1 Francisco Ciruela
YR 2014
UL http://www.jneurosci.org/content/34/44/14793.abstract
AB Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca2+ wave-dependent activation of small-conductance Ca2+-activated K+ (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K+ currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly.