TY - JOUR T1 - Coassembly and Coupling of SK2 Channels and mGlu<sub>5</sub> Receptors JF - The Journal of Neuroscience JO - J. Neurosci. SP - 14793 LP - 14802 DO - 10.1523/JNEUROSCI.2038-14.2014 VL - 34 IS - 44 AU - Gloria García-Negredo AU - David Soto AU - Javier Llorente AU - Xavier Morató AU - Koen M.O. Galenkamp AU - Maricel Gómez-Soler AU - Víctor Fernández-Dueñas AU - Masahiko Watanabe AU - John P. Adelman AU - Ryuichi Shigemoto AU - Yugo Fukazawa AU - Rafael Luján AU - Francisco Ciruela Y1 - 2014/10/29 UR - http://www.jneurosci.org/content/34/44/14793.abstract N2 - Group I metabotropic glutamate (mGlu) receptors regulate hippocampal CA1 pyramidal neuron excitability via Ca2+ wave-dependent activation of small-conductance Ca2+-activated K+ (SK) channels. Here, we show that mGlu5 receptors and SK2 channels coassemble in heterologous coexpression systems and in rat brain. Further, in cotransfected cells or rat primary hippocampal neurons, mGlu5 receptor stimulation activated apamin-sensitive SK2-mediated K+ currents. In addition, coexpression of mGlu5 receptors and SK2 channels promoted plasma membrane targeting of both proteins and correlated with increased mGlu5 receptor function that was unexpectedly blocked by apamin. These results demonstrate a reciprocal functional interaction between mGlu5 receptors and SK2 channels that reflects their molecular coassembly. ER -