RT Journal Article SR Electronic T1 Upregulation of Parkin in Endophilin Mutant Mice JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 16544 OP 16549 DO 10.1523/JNEUROSCI.1710-14.2014 VO 34 IS 49 A1 Cao, Mian A1 Milosevic, Ira A1 Giovedi, Silvia A1 De Camilli, Pietro YR 2014 UL http://www.jneurosci.org/content/34/49/16544.abstract AB Several proteins encoded by PD genes are implicated in synaptic vesicle traffic. Endophilin, a key factor in the endocytosis of synaptic vesicles, was shown to bind to, and be ubiquitinated by, the PD-linked E3 ubiquitin ligase Parkin. Here we report that Parkin's level is specifically upregulated in brain and fibroblasts of endophilin mutant mice due to increased transcriptional regulation. Studies of transfected HEK293T cells show that Parkin ubiquitinates not only endophilin, but also its major binding partners, dynamin and synaptojanin 1. These results converge with the recently reported functional relationship of endophilin to the PD gene LRRK2 and with the identification of a PD-linked synaptojanin 1 mutation, in providing evidence for a link between PD and endocytosis genes.