TY - JOUR T1 - The LIM Homeodomain Factor Lhx2 Is Required for Hypothalamic Tanycyte Specification and Differentiation JF - The Journal of Neuroscience JO - J. Neurosci. SP - 16809 LP - 16820 DO - 10.1523/JNEUROSCI.1711-14.2014 VL - 34 IS - 50 AU - Juan Salvatierra AU - Daniel A. Lee AU - Cristina Zibetti AU - Maria Duran-Moreno AU - Sooyeon Yoo AU - Elizabeth A. Newman AU - Hong Wang AU - Joseph L. Bedont AU - Jimmy de Melo AU - Ana L. Miranda-Angulo AU - Sara Gil-Perotin AU - Jose Manuel Garcia-Verdugo AU - Seth Blackshaw Y1 - 2014/12/10 UR - http://www.jneurosci.org/content/34/50/16809.abstract N2 - Hypothalamic tanycytes, a radial glial-like ependymal cell population that expresses numerous genes selectively enriched in embryonic hypothalamic progenitors and adult neural stem cells, have recently been observed to serve as a source of adult-born neurons in the mammalian brain. The genetic mechanisms that regulate the specification and maintenance of tanycyte identity are unknown, but are critical for understanding how these cells can act as adult neural progenitor cells. We observe that LIM (Lin-11, Isl-1, Mec-3)-homeodomain gene Lhx2 is selectively expressed in hypothalamic progenitor cells and tanycytes. To test the function of Lhx2 in tanycyte development, we used an intersectional genetic strategy to conditionally delete Lhx2 in posteroventral hypothalamic neuroepithelium, both embryonically and postnatally. We observed that tanycyte development was severely disrupted when Lhx2 function was ablated during embryonic development. Lhx2-deficient tanycytes lost expression of tanycyte-specific genes, such as Rax, while also displaying ectopic expression of genes specific to cuboid ependymal cells, such as Rarres2. Ultrastructural analysis revealed that mutant tanycytes exhibited a hybrid identity, retaining radial morphology while becoming multiciliated. In contrast, postnatal loss of function of Lhx2 resulted only in loss of expression of tanycyte-specific genes. Using chromatin immunoprecipitation, we further showed that Lhx2 directly regulated expression of Rax, an essential homeodomain factor for tanycyte development. This study identifies Lhx2 as a key intrinsic regulator of tanycyte differentiation, sustaining Rax-dependent activation of tanycyte-specific genes while also inhibiting expression of ependymal cell-specific genes. These findings provide key insights into the transcriptional regulatory network specifying this still poorly characterized cell type. ER -