PT - JOURNAL ARTICLE AU - Alfredo Gonzalez-Sulser AU - Daniel Parthier AU - Antonio Candela AU - Christina McClure AU - Hugh Pastoll AU - Derek Garden AU - Gülşen Sürmeli AU - Matthew F. Nolan TI - GABAergic Projections from the Medial Septum Selectively Inhibit Interneurons in the Medial Entorhinal Cortex AID - 10.1523/JNEUROSCI.1612-14.2014 DP - 2014 Dec 10 TA - The Journal of Neuroscience PG - 16739--16743 VI - 34 IP - 50 4099 - http://www.jneurosci.org/content/34/50/16739.short 4100 - http://www.jneurosci.org/content/34/50/16739.full SO - J. Neurosci.2014 Dec 10; 34 AB - The medial septum (MS) is required for theta rhythmic oscillations and grid cell firing in the medial entorhinal cortex (MEC). While GABAergic, glutamatergic, and cholinergic neurons project from the MS to the MEC, their synaptic targets are unknown. To investigate whether MS neurons innervate specific layers and cell types in the MEC, we expressed channelrhodopsin-2 in mouse MS neurons and used patch-clamp recording in brain slices to determine the response to light activation of identified cells in the MEC. Following activation of MS axons, we observed fast monosynaptic GABAergic IPSPs in the majority (>60%) of fast-spiking (FS) and low-threshold-spiking (LTS) interneurons in all layers of the MEC, but in only 1.5% of nonstellate principal cells (NSPCs) and in no stellate cells. We also observed fast glutamatergic responses to MS activation in a minority (<5%) of NSPCs, FS, and LTS interneurons. During stimulation of MS inputs at theta frequency (10 Hz), the amplitude of GABAergic IPSPs was maintained, and spike output from LTS and FS interneurons was entrained at low (25–60 Hz) and high (60–180 Hz) gamma frequencies, respectively. By demonstrating cell type-specific targeting of the GABAergic projection from the MS to the MEC, our results support the idea that the MS controls theta frequency activity in the MEC through coordination of inhibitory circuits.