PT  - JOURNAL ARTICLE
AU  - Forrest Collman
AU  - JoAnn Buchanan
AU  - Kristen D. Phend
AU  - Kristina D. Micheva
AU  - Richard J. Weinberg
AU  - Stephen J Smith
TI  - Mapping Synapses by Conjugate Light-Electron Array Tomography
AID  - 10.1523/JNEUROSCI.4274-14.2015
DP  - 2015 Apr 08
TA  - The Journal of Neuroscience
PG  - 5792--5807
VI  - 35
IP  - 14
4099  - http://www.jneurosci.org/content/35/14/5792.short
4100  - http://www.jneurosci.org/content/35/14/5792.full
SO  - J. Neurosci.2015 Apr 08; 35
AB  - Synapses of the mammalian CNS are diverse in size, structure, molecular composition, and function. Synapses in their myriad variations are fundamental to neural circuit development, homeostasis, plasticity, and memory storage. Unfortunately, quantitative analysis and mapping of the brain's heterogeneous synapse populations has been limited by the lack of adequate single-synapse measurement methods. Electron microscopy (EM) is the definitive means to recognize and measure individual synaptic contacts, but EM has only limited abilities to measure the molecular composition of synapses. This report describes conjugate array tomography (AT), a volumetric imaging method that integrates immunofluorescence and EM imaging modalities in voxel-conjugate fashion. We illustrate the use of conjugate AT to advance the proteometric measurement of EM-validated single-synapse analysis in a study of mouse cortex.