TY - JOUR T1 - Mapping Synapses by Conjugate Light-Electron Array Tomography JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5792 LP - 5807 DO - 10.1523/JNEUROSCI.4274-14.2015 VL - 35 IS - 14 AU - Forrest Collman AU - JoAnn Buchanan AU - Kristen D. Phend AU - Kristina D. Micheva AU - Richard J. Weinberg AU - Stephen J Smith Y1 - 2015/04/08 UR - http://www.jneurosci.org/content/35/14/5792.abstract N2 - Synapses of the mammalian CNS are diverse in size, structure, molecular composition, and function. Synapses in their myriad variations are fundamental to neural circuit development, homeostasis, plasticity, and memory storage. Unfortunately, quantitative analysis and mapping of the brain's heterogeneous synapse populations has been limited by the lack of adequate single-synapse measurement methods. Electron microscopy (EM) is the definitive means to recognize and measure individual synaptic contacts, but EM has only limited abilities to measure the molecular composition of synapses. This report describes conjugate array tomography (AT), a volumetric imaging method that integrates immunofluorescence and EM imaging modalities in voxel-conjugate fashion. We illustrate the use of conjugate AT to advance the proteometric measurement of EM-validated single-synapse analysis in a study of mouse cortex. ER -