RT Journal Article
SR Electronic
T1 Clathrin-Independent Trafficking of AMPA Receptors
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 4830
OP 4836
DO 10.1523/JNEUROSCI.3571-14.2015
VO 35
IS 12
A1 Glebov, Oleg O.
A1 Tigaret, Cezar M.
A1 Mellor, Jack R.
A1 Henley, Jeremy M.
YR 2015
UL http://www.jneurosci.org/content/35/12/4830.abstract
AB Membrane trafficking of AMPA receptors (AMPARs) is critical for neuronal function and plasticity. Although rapid forms of AMPAR internalization during long-term depression (LTD) require clathrin and dynamin, the mechanisms governing constitutive AMPAR turnover and internalization of AMPARs during slow homeostatic forms of synaptic plasticity remain unexplored. Here, we show that, in contrast to LTD, constitutive AMPAR internalization and homeostatic AMPAR downscaling in rat neurons do not require dynamin or clathrin function. Instead, constitutive AMPAR trafficking is blocked by a Rac1 inhibitor and is regulated by a dynamic nonstructural pool of F-actin. Our findings reveal a novel role for neuronal clathrin-independent endocytosis controlled by actin dynamics and suggest that the interplay between different modes of receptor endocytosis provides for segregation between distinct modes of neuronal plasticity.