PT  - JOURNAL ARTICLE
AU  - Quentin Chevy
AU  - Martin Heubl
AU  - Marie Goutierre
AU  - Stéphanie Backer
AU  - Imane Moutkine
AU  - Emmanuel Eugène
AU  - Evelyne Bloch-Gallego
AU  - Sabine Lévi
AU  - Jean Christophe Poncer
TI  - KCC2 Gates Activity-Driven AMPA Receptor Traffic through Cofilin Phosphorylation
AID  - 10.1523/JNEUROSCI.1735-15.2015
DP  - 2015 Dec 02
TA  - The Journal of Neuroscience
PG  - 15772--15786
VI  - 35
IP  - 48
4099  - http://www.jneurosci.org/content/35/48/15772.short
4100  - http://www.jneurosci.org/content/35/48/15772.full
SO  - J. Neurosci.2015 Dec 02; 35
AB  - Expression of the neuronal K/Cl transporter KCC2 is tightly regulated throughout development and by both normal and pathological neuronal activity. Changes in KCC2 expression have often been associated with altered chloride homeostasis and GABA signaling. However, recent evidence supports a role of KCC2 in the development and function of glutamatergic synapses through mechanisms that remain poorly understood. Here we show that suppressing KCC2 expression in rat hippocampal neurons precludes long-term potentiation of glutamatergic synapses specifically by preventing activity-driven membrane delivery of AMPA receptors. This effect is independent of KCC2 transporter function and can be accounted for by increased Rac1/PAK- and LIMK-dependent cofilin phosphorylation and actin polymerization in dendritic spines. Our results demonstrate that KCC2 plays a critical role in the regulation of spine actin cytoskeleton and gates long-term plasticity at excitatory synapses in cortical neurons.SIGNIFICANCE STATEMENT Changes in the expression of neuronal chloride transporters, such as KCC2, occur during postnatal development and are induced in a variety of neurological and psychiatric conditions. Such changes are expected to primarily impact GABA signaling because GABAA receptors are predominantly permeable to chloride ions. However, the KCC2 transporter forms clusters near glutamatergic synapses and interacts with several actin-related proteins. We show that KCC2 is strictly required for LTP expression at hippocampal excitatory synapses. This effect is due to KCC2 interaction with the Rac1/PAK signaling pathway that controls actin polymerization. Suppressing this interaction promotes actin polymerization thereby hindering AMPA receptor traffic upon KCC2 suppression. Alterations of KCC2 expression therefore impact not only GABAergic signaling but also glutamatergic synaptic function and long term plasticity.