RT Journal Article
SR Electronic
T1 Experience-Dependent Accumulation of <em>N</em><sup>6</sup>-Methyladenosine in the Prefrontal Cortex Is Associated with Memory Processes in Mice
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 6771
OP 6777
DO 10.1523/JNEUROSCI.4053-15.2016
VO 36
IS 25
A1 Widagdo, Jocelyn
A1 Zhao, Qiong-Yi
A1 Kempen, Marie-Jeanne
A1 Tan, Men Chee
A1 Ratnu, Vikram S.
A1 Wei, Wei
A1 Leighton, Laura
A1 Spadaro, Paola A.
A1 Edson, Janette
A1 Anggono, Victor
A1 Bredy, Timothy W.
YR 2016
UL http://www.jneurosci.org/content/36/25/6771.abstract
AB The RNA modification N6-methyladenosine (m6A) influences mRNA stability and cell-type-specific developmental programming, and is highly abundant in the adult brain. However, it has not been determined whether m6A is dynamically regulated by experience. Based on transcriptome-wide profiling of m6A, we report that the level of m6A increases in the medial prefrontal cortex (mPFC) of mice in response to behavioral experience. The modulation was enriched near the stop codon of mRNAs, including genes related to neuronal plasticity. In primary cortical neurons, in vitro, modulation of m6A by the RNA demethylase FTO influenced the degradation profiles of a subset of transcripts with modulated sites. In vivo, the expression of Fto and the m6A methyltransferase, Mettl3 correlated with the observed increase in m6A levels post-training. Furthermore, targeted knockdown of FTO in the mPFC led to enhanced consolidation of cued fear memory. Thus, together with its role in early development, the dynamic regulation of m6A in the adult brain serves as an important epitranscriptomic mechanism associated with behavioral adaptation.SIGNIFICANCE STATEMENT N6-methyladenosine (m6A) is the most prevalent internal modification on RNA, however, its cellular dynamics in vivo remains elusive. Here we provide the first demonstration of m6A upregulation in the mouse medial prefrontal cortex (mPFC) following behavioral training. Knocking down the m6A demethylase FTO in the mPFC, which increases total m6A level, results in enhanced consolidation of fear memory. Our findings suggest that m6A is regulated in an activity-dependent manner in the adult brain, and may function to fine-tune mRNA turnover during memory-related processes.