TY - JOUR T1 - Increasing central serotonin with 5-HTP disrupts the inhibition of social gaze in non-human primates JF - The Journal of Neuroscience JO - J. Neurosci. DO - 10.1523/JNEUROSCI.0413-21.2021 SP - JN-RM-0413-21 AU - Hannah Weinberg-Wolf AU - Nicholas A. Fagan AU - Olga Dal Monte AU - Steve W. C. Chang Y1 - 2021/12/03 UR - http://www.jneurosci.org/content/early/2021/12/02/JNEUROSCI.0413-21.2021.abstract N2 - To competently navigate the world, individuals must flexibly balance distinct aspects of social gaze, orienting toward others and inhibiting orienting responses, depending on the context. These behaviors are often disrupted in patient populations treated with serotonergic drugs. However, the field lacks a clear understanding of how the serotonergic system mediates social orienting and inhibiting behaviors. Here, we tested how increasing central concentrations of serotonin with the direct precursor 5-Hydroxytryptophan (5-HTP) would modulate the ability of rhesus macaques (both sexes) to use eye movements to flexibly orient to, or inhibit orienting to, faces. Systemic administrations of 5-HTP effectively increased central serotonin levels and impaired flexible orientation and inhibition. Critically, 5-HTP selectively impaired the ability of monkeys to inhibit orienting to face images, whereas it similarly impaired orienting to face and control images. 5-HTP also caused monkeys to perseverate on their gaze responses, making them worse at flexibly switching between orientating and inhibiting behaviors. Furthermore, the effects of 5-HTP on performance correlated with a constriction of the pupil, an increased time to initiate trials, and an increased reaction time, suggesting that the disruptive effects of 5-HTP on social gaze behaviors are likely driven by a downregulation of arousal and motivational states. Taken together, these findings provide causal evidence for a modulatory relationship between 5-HTP and social gaze behaviors in non-human primates and offer translational insights for the role of the serotonergic system in social gaze.SignificanceBehavioral changes arising from pharmacological agents that target serotonergic functions are complex and difficult to predict. Here, we examined the causal impacts of administering serotonin’s direct precursor, 5-Hydroxytroptohan (5-HTP), on orienting and inhibiting social gaze in non-human primates. 5-HTP increased central concentrations of serotonin and selectively impaired the ability of monkeys to inhibit orienting to faces while similarly impairing monkeys’ ability to orient to face and control images. These behavioral gaze impairments were systematically associated with a downregulation of arousal and motivational states, indexed by constriction of the pupil, increased time to initiate trials, and increased reaction time. These findings provide a causal link between 5-HTP and social gaze behaviors in non-human primates and provide translational insights about serotonergic interventions. ER -