RT Journal Article
SR Electronic
T1 Visual Deprivation during Mouse Critical Period Reorganizes Network-Level Functional Connectivity
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP e1019232024
DO 10.1523/JNEUROSCI.1019-23.2024
VO 44
IS 19
A1 Chen, Siyu
A1 Rahn, Rachel M.
A1 Bice, Annie R.
A1 Bice, Seana H.
A1 Padawer-Curry, Jonah A.
A1 Hengen, Keith B.
A1 Dougherty, Joseph D.
A1 Culver, Joseph P.
YR 2024
UL http://www.jneurosci.org/content/44/19/e1019232024.abstract
AB A classic example of experience-dependent plasticity is ocular dominance (OD) shift, in which the responsiveness of neurons in the visual cortex is profoundly altered following monocular deprivation (MD). It has been postulated that OD shifts also modify global neural networks, but such effects have never been demonstrated. Here, we use wide-field fluorescence optical imaging (WFOI) to characterize calcium-based resting-state functional connectivity during acute (3 d) MD in female and male mice with genetically encoded calcium indicators (Thy1-GCaMP6f). We first establish the fundamental performance of WFOI by computing signal to noise properties throughout our data processing pipeline. Following MD, we found that Δ band (0.4–4 Hz) GCaMP6 activity in the deprived visual cortex decreased, suggesting that excitatory activity in this region was reduced by MD. In addition, interhemispheric visual homotopic functional connectivity decreased following MD, which was accompanied by a reduction in parietal and motor homotopic connectivity. Finally, we observed enhanced internetwork connectivity between the visual and parietal cortex that peaked 2 d after MD. Together, these findings support the hypothesis that early MD induces dynamic reorganization of disparate functional networks including the association cortices.