PT - JOURNAL ARTICLE AU - Fernandes-Henriques, Carolina AU - Guetta, Yuval AU - Sclar, Mia G. AU - Zhang, Rebecca AU - Miura, Yuka AU - Surrence, Katherine R. AU - Friedman, Allyson K. AU - Likhtik, Ekaterina TI - Infralimbic Projections to the Substantia Innominata–Ventral Pallidum Constrain Defensive Behavior during Extinction Learning AID - 10.1523/JNEUROSCI.1001-24.2025 DP - 2025 May 28 TA - The Journal of Neuroscience PG - e1001242025 VI - 45 IP - 22 4099 - http://www.jneurosci.org/content/45/22/e1001242025.short 4100 - http://www.jneurosci.org/content/45/22/e1001242025.full SO - J. Neurosci.2025 May 28; 45 AB - Fear extinction is critical for decreasing fear responses to a stimulus that is no longer threatening. While it is known that the infralimbic (IL) region of the medial prefrontal cortex mediates retrieval of an extinction memory through projections to the basolateral amygdala (BLA), IL pathways contributing to extinction learning are not well understood. Given the dense projection from the IL to the substantia innominata–ventral pallidum (SI/VP), an area that processes aversive and appetitive cues, we compared how the IL→SI/VP functions in extinction compared with the IL→BLA pathway in male mice. Using retrograde tracing, we demonstrate that IL projections to the SI/VP originate from superficial [Layer (L)2/3] and deep cortical layers (L5) and that they are denser than IL projections to the BLA. Next, combining retrograde tracing with labeling for the immediate early gene cFos, we show increased activity of L5 IL→SI/VP output during extinction learning and increased activity of L2/3 IL→BLA output during extinction retrieval. Then, using in vitro recordings, we demonstrate that neurons in the IL→SI/VP pathway are more excitable during extinction learning than retrieval. Finally, using optogenetics, we inactivate the IL→SI/VP pathway and show that this increases defensive freezing during extinction learning and re-extinction, without affecting memory. Taken together, we demonstrate that the IL→SI/VP pathway is active during extinction learning, when it constrains the defensive freezing response. We propose that the IL acts as a switchboard operator, increasing IL L5 communication with the SI/VP during extinction learning and IL L2/3 communication with the BLA during extinction retrieval.