Regional distribution of basal and NADH-enhanced [3H]DHR binding
| Region | Basal | Enhanced | % Enhancement |
|---|---|---|---|
| Olfactory bulb | |||
| Glomerular layer | 8.17 ± 1.77 (24) | 266.1 ± 19.2 (59) | 3157 |
| External plexiform layer | 34.8 ± 7.12 (100) | 452.9 ± 26.4 (100) | 1201 |
| Internal granular layer | 13.4 ± 1.74 (39) | 157.6 ± 11.3 (39) | 1073 |
| Basal ganglia | |||
| Striatum | 9.16 ± 1.18 (26) | 132.8 ± 9.43 (29) | 1350 |
| Globus pallidus | 3.35 ± 0.28 (10) | 38.10 ± 3.70 (8) | 1037 |
| Substantia nigra | 4.08 ± 1.58 (12) | 131.0 ± 13.0 (29) | 3111 |
| Cerebral cortex | |||
| Parietal, outer | 17.9 ± 2.85 (52) | 227.7 ± 9.65 (50) | 1170 |
| Parietal, inner | 10.6 ± 0.88 (31) | 88.11 ± 7.93 (20) | 730 |
| Septal region | |||
| Lateral septal nucleus | 21.5 ± 3.33 (62) | 189.6 ± 11.0 (42) | 783 |
| Diagonal band | 8.25 ± 4.52 (24) | 129.1 ± 10.8 (29) | 1465 |
| Nucleus accumbens | ND | 219.8 ± 5.85 (49) | — |
| Thalamus | |||
| Reticular | 6.88 ± 3.22 (20) | 71.80 ± 8.68 (16) | 944 |
| Ventroposteromedial | 13.0 ± 10.0 (37) | 130.0 ± 17.6 (29) | 900 |
| Laterodorsal | 4.76 ± 1.65 (14) | 175.8 ± 23.0 (39) | 3592 |
| Habenula | 6.48 ± 1.91 (19) | 110.0 ± 15.7 (24) | 1597 |
| Medial geniculate | 2.22 ± 1.19 (6) | 115.1 ± 19.5 (25) | 5086 |
| Hippocampus | |||
| CA1 | |||
| Stratum moleculare | 24.2 ± 3.95 (70) | 218.0 ± 23.7 (48) | 799 |
| Stratum radiatum | 14.1 ± 2.44 (41) | 229.0 ± 28.8 (51) | 1519 |
| Stratum pyramidale | 13.2 ± 3.24 (38) | 63.4 ± 12.9 (14) | 381 |
| Stratum oriens | 12.5 ± 3.10 (36) | 217.9 ± 16.7 (48) | 1636 |
| CA2 | 16.7 ± 3.91 (48) | 220.7 ± 18.7 (49) | 1218 |
| CA3 | 12.9 ± 3.73 (37) | 244.1 ± 26.2 (54) | 1795 |
| Dentate gyrus | 21.2 ± 4.22 (61) | 327.1 ± 29.8 (72) | 1446 |
| Forebrain, other | |||
| Hypothalamus | 7.76 ± 3.31 (22) | 157.6 ± 9.24 (35) | 1932 |
| Amygdala, basolateral | 13.1 ± 3.87 (38) | 127.2 ± 17.8 (28) | 869 |
| Brainstem | |||
| Superior colliculus | |||
| Superficial gray layer | 10.0 ± 3.60 (29) | 199.6 ± 24.0 (44) | 1904 |
| Optic nerve layer | 2.38 ± 1.69 (7) | 82.56 ± 15.9 (18) | 3369 |
| Periaqueductal gray | ND | 120.7 ± 23.0 (27) | — |
| Interpeduncular nucleus | 10.1 ± 4.56 (29) | 245.9 ± 10.2 (54) | 2334 |
| Oculomotor nucleus | ND | 244.5 ± 15.8 (54) | — |
| Facial nucleus | 6.82 ± 1.08 (20) | 216.3 ± 28.5 (48) | 3072 |
| Hypoglossal nucleus | ND | 236.8 ± 18.2 (52) | — |
| Cerebellum | |||
| Molecular layer | 3.61 ± 0.08 (10) | 283.4 ± 35.0 (63) | 7750 |
| Granular layer | 3.52 ± 1.61 (10) | 167.8 ± 15.5 (37) | 4668 |
| Kidney | |||
| Cortex | 161.1 ± 19.9 (463) | 451.6 ± 15.5 (100) | 174 |
| Medulla | 369.8 ± 26.7 (1060) | 571.8 ± 10.5 (126) | 55 |
| Muscle | |||
| Striated | 163.6 ± 94.3 (470) | 207.9 ± 76.3 (46) | 27 |
| Cardiac | 315.9 ± 17.1 (908) | 544.3 ± 11.0 (120) | 72 |
Enhanced binding was performed in the presence of 200 μm NADH; [3H]DHR concentration was 5 nm. Binding is in units of fmol/mg tissue; values represent mean ± SEM (n = 4). ND, Not distinguishable from background (% external plexiform layer binding).