Effects of GABA, baclofen, picrotoxin, cAMP analog, IBMX, PKAI, and PKCI on calcium currents
| Control | Experimental | df | t | p | ||
|---|---|---|---|---|---|---|
| Treatment | nA | Treatment | nA | |||
| — | −0.95 ± 0.29 (7) | GABA* (10 nm) | −1.09 ± 0.38 (7) | 6 | 2.5 | <0.05 |
| — | −0.17 ± 0.06 (7) | GABAI,t (10 nm) | −0.23 ± 0.04 (7) | 6 | 3.1 | <0.025 |
| — | −0.94 ± 0.30 (7) | GABA* (30 μm) | −0.67 ± 0.20 (7) | 6 | 3.0 | <0.025 |
| — | −1.01 ± 0.11 (9) | Baclofen (25 μm) | −0.66 ± 0.27 (9) | 8 | 3.4 | <0.01 |
| GABA (50 nm) | −0.78 ± 0.21 (6) | GABA (50 nm) + Picro (1 μm) | −0.19 ± 0.16 (6) | 5 | 9.5 | <0.005 |
| — | −1.28 ± 0.16 (7) | Dibu.cAMP (2.5 nm) | −2.17 ± 0.33 (7) | 6 | 7.5 | <0.005 |
| — | −0.90 ± 0.18 (5) | IBMX (200 μm) | −1.16 ± 0.21 (5) | 4 | 4.3 | <0.025 |
| PKAI (5 μm) | −1.00 ± 0.21 (5) | PKAI (5 μm) + GABA (50 nm) | −0.43 ± 0.13 (5) | 4 | 4.8 | <0.01 |
| PKCI (5 μm) | −1.08 ± 0.26 (6) | PKCI (5 μm) + GABA (25 μm) | −0.57 ± 0.12 (6) | 5 | 3.7 | <0.025 |
Asterisk indicates that the Vh was −30 mV and step potential was +20 mV. Vh for other experiments was −80 mV, and the step voltage was −20 mV. The transient component of the calcium current (It) was elicited from a holding potential of −80 mV to step potentials of −10 mV. Data are expressed as the mean peak current ± SD (number of cells). Computed t statistics are for tests of correlated means. Picro, Picrotoxin; dibu.cAMP, cAMP-N6,O6-dibutyril.