Growth cone morphology and motility of normal and mutant neurons in culture
| Genotype | Growth cone morphology | Growth cone motility | |||||
|---|---|---|---|---|---|---|---|
| Filopodia | Lamellipodia | n | |||||
| No/GC ± SEM | Length ± SEM | Area ± SEM | Roundness ± SEM | Index ± SD | n | ||
| Normal | 3.1 ± 0.2 | 5.0 ± 0.3 | 18.3 ± 1.0 | 0.38 ± 0.02 | 53 | 0.36 ± 0.09 | 5 |
| dnc1 | 3.1 ± 0.2 | 4.7 ± 0.3 | 17.7 ± 1.7 | 0.40 ± 0.16 | 58 | 0.10 ± 0.03* | 4 |
| dncM11 | 3.5 ± 0.3 | 5.2 ± 0.4 | 16.2 ± 2.5 | 0.37 ± 0.02 | 39 | 0.09 ± 0.03* | 4 |
| dncM14 | 3.3 ± 0.7 | 4.6 ± 0.5 | 15.5 ± 1.8 | 0.29 ± 0.09 | 10 | 0.07 ± 0.03* | 4 |
| rut1 | 2.9 ± 0.2 | 5.2 ± 0.4 | 13.4 ± 0.9 | 0.41 ± 0.02 | 60 | 0.07 ± 0.03* | 4 |
| dnc1rut1 | 3.2 ± 0.6 | 4.9 ± 0.7 | 18.2 ± 2.0 | ND | 20 | 0.36 ± 0.08 | 5 |
Nerve growth cones were observed between 16 and 48 hr after plating on uncoated glass coverslips. Filopodial length is given in micrometers and lamellipodial area in micrometers squared. For roundness and motility index, see text. The data include only growth cones with phase-light lamellipodia in type III monopolar neurons. *p < 0.001. ND, Not determined.